Advanced/optional: Anastomotic concept and anastomosis – 120 degrees, 9 stitches
The rat abdominal aorta can also be anastomosed using only nine stitches, providing that that they are perfectly spaced. Because of the high degree of precision needed to obtain an anastomosis that does not leak, we recommend mastering a good 12-stitch anastomosis first.
Place stitches one, two and five 120 degrees apart. Stitches three and four, six and seven and eight and nine are placed in pairs in their respective 120-degree segments.
The same concept is presented more extensively in the femoral artery and vein exercises.
If your anastomosis is patent, cover it up with moist gauze and leave it alone for 20 minutes. Then test its patency once more.
If you have handled the abdominal aorta gently and assume damage to the intimal layer to be minimal you can perform further anastomoses on it for as often as you can approximate the vessel ends.
If the anastomosis is patent you can also work on the femoral vessels or inferiorvena cava. If it is not patent you can work on the neck vessels.